Study on typing of DNA extracted from urine and urine stains
- VernacularTitle:尿液及尿斑DNA分型的研究
- Author:
Ronghua CHEN
;
Lihua GU
;
Yuan PING
- Publication Type:Journal Article
- Keywords:
Forensic physical evidence;
Urine;
Urine stains;
QIAamp Mini Kit;
PCR;
STR typing
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the extraction of DNA from urine and urine stains and typing.Methods DNA was extracted from urine and urine stains with Chelex-100 extraction method and QIAamp Mini Kit.STR loci were typed after amplification by PCR procedures with Profiler Plus Kit.Results STR loci could be well typed in DNA extracted from the urine,which was fresh or preserved properly for less than 12 hours.Satisfactory DNA typing could be made in 50% of the urine preserved for two days.No typing results were obtained in the samples kept for seven days or much longer time,and typing was rarely available using urine stains.Conclusion DNA extracted from the fresh urine can be typed,which may be used in forensic identification.
