Effect of exogenous prostaglandin E_1 on expression of platelet-derived growth factor B and its receptor ? in rabbits with schistosomia japonicum
- VernacularTitle:外源性前列腺素E_1对血吸虫病家兔肝脏血小板衍生生长因子B及其受体表达的影响
- Author:
Weilong ZOU
;
Zhen YANG
;
Zhipeng LIANG
;
Dongjian LI
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis;
Alprostadil;
Platelet-derived growth factor;
Hepatic stellate cell
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of exogenous prostaglandin E 1 (PGE 1) on expression of platelet derived growth factor B (PDGF-B) mRNA and its receptor ? protein in rabbit with schistosomiasis. Methods In this study, 14 rabbits were infected with cercaria of S. japonicum percutaneously. PGE 1 ( 2.5 ?g/kg?d -1 ) was given intravenously to 7 rabbits from the 60th day to day 120. The expressions of PDGF-B mRNA, PDGFR ? protein and ?-SMA were detected by RT-PCR, Western blotting and immunohistochemistry, respectively. Endogenous IFN-? was measured by in situ hybridization. Results Up-regulated expressions of PDGF-B mRNA, receptor ? protein as well as ?-SMA were observed in rabbits with Schistosome hepatic fibrosis. The increased expressions of PDGF mRNA and receptor ? were suppressed in rabbits treated with exogenous PGE 1 (29.42?5.05 vs 41.37?7.23, P
