The Study of WT1 Gene Expression in the Urine from Patients with Renal Diseases by Semi-nested PCR
- VernacularTitle:半嵌套式RT-PCR法检测肾病患者尿沉渣中WT1基因表达研究
- Author:
Ming GAO
;
Aiguo TAN
;
Jianping CAI
- Publication Type:Journal Article
- Keywords:
Semi-nested PCR;
Gene expression;
Diabetes mellitus
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of WT1 gene expression in the urine. Methods The expression of WT1 gene in the urine of patients with chronic glomerular nephritis(CGN), rheumatic diseases,diabetes mellitus(DM) and healthy subjects was detected by semi-nested PCR. Results The positive rates of WT1 gene expression in CGN with proteinuria,DM with proteinuria, DM without proteinuria and rheumatic diseases were 46.7% (14/30),44.4% (16/36),5.95% (5/84) and 16%(4/25), respectively. WT1 expression in the urine was not related to the degree of proteinuria, hematuria, renal function or clinical course of diseases. WT1 expression in the urine of the healthy subjects or cystitis patients was negative. Conclusion The measurement of WT1 mRNA in the urine could play an important role in early diagnosis of progressive renal damage.
