Inhibitory Effects of Ligustrazine on the Expression of Precollagen I,III Induced by Umbilical Serum of Preeclampsia in Cultured Human Umbilical Artery Smooth Muscle Cell
- VernacularTitle:川芎嗪对子痫前期脐血清诱导脐动脉平滑肌细胞胶原表达的体外研究
- Author:
Rongzhen JIANG
;
Hanping CHEN
- Publication Type:Journal Article
- Keywords:
Preeclampsia;
Human umbilical artery smooth muscle cell;
Ligustrazine
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of ligustrazine(LTZ) on the preeclampyic umbilical serum induced-expression of precollagen I,III in cultured human umbilical artery smooth muscle cell(HUSMC). Methods The cultured HUSMC was treated with LTZ for 30 min, and then incubated with medium containing 20% serum either from women with preeclampsia or normal pregnant women for 48h. The cell activity was determined by MTT, the cell cycle was analyzed by flow cytomerty, and RT-PCR analysis was used to dectect the expression of precollagen I,III. Results The cell proliferation, percentage of S and G2/M phases, and expression of precollagen I obviously increased in HUSMC incubated with preeclampsia umbilical serum compared with normal pregnant women one(P
