The Study of Cell-cycle Specificity of Tamoxifen-induced Apoptosis in the ER(+) Breast Cancer Cells
- VernacularTitle:他莫昔芬诱导体内外雌激素受体阳性乳腺癌细胞凋亡的周期特异性研究
- Author:
Ziming HUANG
;
Lin ZHANG
;
Huiling YUAN
- Publication Type:Journal Article
- Keywords:
Breast neoplasm;
Cell-cycle;
Apoptosis;
Tamoxifen;
Cell culture
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cell-cycle specificity of tamoxifen-induced apoptosis in the ER(+) breast cancer cells in vitro and in vivo. Methods ER(+) MCF-7 cell line (in vitro) and primary cultured ER(+) breast cancer cells (in vivo) were treated with tamoxifen, and the cell cycle specificity of cell apoptosis and the apoptotic rate were dertermined by flow cytometry. Results Both MCF-7 and primary cultured breast cancer cells were induced to apoptosis with G 0/G 1 phase specificity by tamoxifen. And the apoptotic rate in MCF-7 was higher than that in primary cultured breast cancer cells. Conclusion Tamoxifen could induced G 0/G 1 phase specific apoptosis in the ER(+) breast cnacer cells, and tamoxifen-induced apoptotic rate was higher in vitro than in vivo.
