Content Determination of Bacterial Endotoxin in Puerarin and Glucose Injection by Kinetic-turbidimetric Technique
- VernacularTitle:动态比浊法测定葛根素葡萄糖注射液中细菌内毒素的含量
- Author:
Xueqin ZHOU
;
Changjie GUO
- Publication Type:Journal Article
- Keywords:
Puerarin and glucose injection;
Kinetic-turbidimetric;
Bacterial endotoxin;
Interference test
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of bacterial endotoxin in Puerarin and Glucose injection.METHODS:The kinetic-turbidimetric technique was adopted to dilute the puerarin glucose injection by2-fold,in which,the contents of bacterial endotoxin was determinated by preliminary interference test and interference test.RESULTS:Diluting the sample by2-fold could effectively eliminate its interference to tachypleus amebocyte lysate(TAL).The average recovery rate was at the range of50%～200%.CONCLUSLON:Compared with the pyrogen rabbit de-termination method and the half-quantity gel method,the kinetic-turbidimetic technique has many merits such as high de-termination sensitivity,short cycle,accurate result and better reproduction quality,etc.
