Content Determination of 2 Components in Tetracaine Hydrochloride Mucilage by RP-HPLC
- VernacularTitle:反相高效液相色谱法同时测定盐酸丁卡因胶浆中2组分的含量
- Author:
Guifu MAO
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Tetracaine hydrochloride;
Ethyl hydroxybenzoate;
Content determination
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of tetracaine hydrochloride and ethyl hy-droxybenzoate in tetracaine hydrochloride mucilage by RP-HPLC.METHODS:C 8 was the chromatographic column,the mobile phase was methanol-absolute ethanol-water-triethylamine(70∶15∶15∶0.05),the detection wavelength was at280nm.RESULTS:The linear ranges of tetracaine hydrochloride and ethyl hydroxybenzoate were10～50?g/ml(r=0.9997)and2～10?g/ml(r=0.9999)respectively;the average recovery rate were100.82%and100.16%respectively.CONCLUSIONS:The method was simple,rapid and accurate,which can be used for the quality control of tetracaine hydrochloride mucilage.
