Immunologic Characterization of a Genetically Engineered Human Antibody Against Keratin
- VernacularTitle:对一株基因工程人抗角蛋白抗体的免疫学鉴定
- Author:
Ning LU
;
Gang WANG
;
Yufeng LIU
;
Chengxin LI
;
Wei LI
;
Hailong ZHANG
;
Xiaodong ZHAO
- Publication Type:Journal Article
- Keywords:
Keratin;
Antibody;
Genetic engineering
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To immunologically characterize the binding activity of a genetic engineering human antibody against keratin. Methods The specific anti-keratin Fab was screened from a phage antibody library. After Fab monoclonal antibody was induced by isopropylthio-?-D-galactoside (IPTG), the binding activity with antigens and antigenic specificity of the antibody were verified by ELISA,Western blot, and competitive inhibition ELISA. Results The antibody possessed good antigenic specificity as well as excellent binding activities with antigens, and could recognize 46 000 keratin specifically. It was identified as anti-keratin 17 antibody. Conclusions The immunologic characteristics of the genetic engineering antibody are very good. It is necessary to further study the biological activities and clinical application of it.
