Effects of ropivacaine and bupivacaine on L-type calcium currents in ventricular cardiomyocytes of guinea
- VernacularTitle:罗哌卡因和布比卡因对豚鼠心室肌细胞L-型钙电流的影响
- Author:
Hui WU
;
Lihuan LI
;
Lei CHEN
- Publication Type:Journal Article
- Keywords:
Amides;
Bupivacaine;
Myocardium;
Calcium channels, L-type
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ropivacaine and bupivacaine on L-type calcium current (ICa-L) in ventricular myocytes of guinea pigs and to identify the probable mechanism of their negative inotropic effect on myocardium. Methods Single ventricular myocytes were enzymatically isolated from adult male guinea pigs weighing 300-350 g using the enzymatic dissociation method as described by Liu et al. The whole-cell patch clamp technique was used to record ICa-L Results 100 ?mol?L-1 ropivacaine and bupivacaine decreased the peak current of ICa-L by 37% ? 3% and 42% ? 5% respectively ( P
