Changes in expression of calcium channel ?1B subunit mRNA in dorsal root ganglion neurons in a rat model of neuropathic pain
- VernacularTitle:神经病理痛大鼠背根神经节细胞钙通道?1B亚单位mRNA的表达
- Author:
Fang LUO
;
Haosheng BI
- Publication Type:Journal Article
- Keywords:
Neuralgia;
Ganglion, spinal;
Calcium channel;
RNA, messenger
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the changes in expression of Ca2+ channel ?1B subunit mRNA in dorsal root ganglion (DRG) neurons in a rat model of neuropathic pain. Methods Twenty-four SD rats weighing 160-220g were randomly divided into 3 groups with 8 animals in each group: (1) neuropathic pain group; (2) sham-operated group and (3) control group. In neuropathic pain group the animals were anesthetized with intraperitoneal phenobarbital 50 mg?kg-1 . The left L5 spinal nerve was exposed and ligated and cut. In sham-operated the left L5 spinal nerve was exposed but not ligated and cut. Control group underwent no operation. Bilateral paw withdrawal threshold to von Frey hair stimulation was measured before and 7, 14 days after operation. The animals were killed on the 14th day after operation and the left L5 DRGs were obtained for determination of Ca2+ channel ?1B subunit mRNA by in situ hybridization. Results Mechanical allodynia developed in neuropathic pain group but not in sham-operated group. The expression of Ca2+ channel ?1B subunit mRNA was significantly lower in neuropathic pain group (0.033 ? 0.011) than in sham-operated group (0.065 ? 0.042) and control group (0.066 ? 0.034) (P
