Norcantharidin induces HeLa cells apoptosis through caspases pathway
- VernacularTitle:去甲斑蝥素通过半胱氨酸天冬氨酸酶诱导HeLa细胞凋亡
- Author:
Weiwei AN
;
Minwei WANG
;
Xianfeng GONG
;
Shinichi TASHIRO
;
Satoshi ONODERA
;
Takash IKEJIMA
- Publication Type:Journal Article
- Keywords:
Norcantharidin;
HeLa cells;
Apoptosis;
Caspases
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine the apoptotic pathway of norcantharidin (NCTD)-induced HeLa cells death. METHODS: MTT, photomicroscopical observation, DNA agarose gel electrophoresis, LDH release and Western blot analysis were used. RESULTS: NCTD induced HeLa cells apoptosis and the apoptosis was partially reversed by the inhibitors of caspase-family (-3, -8, -10). The activities of caspase-3, -8 and -9 were significantly increased after treated with NCTD. The expression of the inhibitor of caspase-3 activated DNase (ICAD) was decreased in a time dependent manner. CONCLUSION: NCTD induces HeLa cells apoptosis through activating caspase pathways.
