Effects of 17?-estradiol on the maturation and immunologic function of dendritic cells from human peripheral blood
- VernacularTitle:雌二醇对人树突状细胞成熟和功能的影响
- Author:
Yuan SHEN
;
Yaoying ZENG
;
Jingxian ZHAO
;
Xun JIANG
;
Tong WANG
;
Jingfang DI
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Estradiol;
Immunologic function
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of 17?-estradiol (E 2) on the maturation and immunologic function of dendritic cells from human peripheral blood. METHODS: Cultured DCs were treated with E 2 at doses of 10 -7 mol/L and 10 -6 mol/L. The morphologic changes were observed under the scanning electronic microscope. The immunophenotype of DCs in control and treated groups was analyzed by flowcytometry. IL-12 production in culture supernatant was examined by ELISA assay. The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by incorporation of 3H]-TdR. RESULTS: Compared with control group, DCs cultured in the presence of E 2 displayed less dendritic pseudopod, expressed low levels of MHC-II, CD40, CD80 and CD86, and exhibited weakly activity in stimulating the proliferation of allogeneic T cells and reduction of IL-12 production. CONCLUSION: E 2 exerts a negative effect on the maturation and immunologic function of dendritic cells from human peripheral blood.
