Effects of histamine and hypoxia on eNOS gene expression in cultured porcine pulmonary and aorta endothelial cells
- VernacularTitle:组胺和低氧对猪肺动脉和主动脉内皮细胞eNOS基因表达的影响
- Author:
Jingxia GAO
;
Hong YE
;
Huige LI
;
Dixun WANG
- Publication Type:Journal Article
- Keywords:
Histamine;
Anoxia;
Nitric-oxide synthase;
Gene expression regulation
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of histamine and hypoxia on the expression of eNOS mRNA and protein in cultured porcine pulmonary artery and aorta endothelial cells. METHODS: Semi-quantitative RT-PCR and immuno-cytochemistry were used. RESULTS: (1) Histamine increased eNOS mRNA expression in a dose-and time dependent manner. For pulmonary endothelial cells, the effect reached peak when exposed to 10 -5 mol/L histamine in 24 h. eNOS mRNA level was increased to 178 2%?7 7% ( P
