Native and oxidized low density and very low density lipoprotein enhance the expression of MIP-1? mRNA in aortic smooth muscle cells
- VernacularTitle:天然及氧化低密度和极低密度脂蛋白促进动脉平滑肌细胞表达巨噬细胞炎性蛋白1? mRNA
- Author:
Zhiling QU
;
Qiurong RUAN
;
Dahe ZHU
- Publication Type:Journal Article
- Keywords:
Lipoproteins;
Macrophage inflammatory protein-1;
Muscle, smooth, vascular;
Arteriosclerosis
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To understand whether native and oxidized low density and very low density lipoprotein (n-LDL, n-VLDL, ox-LDL, ox-VLDL) enhance the expression of macrophage inflammatory protein (MIP)1? mRNA in cultured aortic smooth muscle cells (SMCs). METHODS: Native low density and very low density lipoprotein were isolated from normal blood donors by density gradient ultracentrifugation, and were oxidatively modified by adding CuCl 2. After a 24 h-exposure of the cultured SMCs to n-LDL, n-VLDL, ox-LDL and ox-VLDL, respectively, the expression of MIP-1? mRNA was determined by in situ hybridization and RT-PCR. RESULTS: Cultured aortic SMCs expressed MIP-1? mRNA at low level. N-LDL, n-VLDL, ox-LDL and ox-VLDL enhanced the expression of MIP-1? mRNA in SMCs, ox-LDL and ox-VLDL showed stronger effect than n-LDL and n-VLDL, respectively. The effect of ox-VLDL was most striking. There was a significant difference between groups ( P
