NF-?B activation participates in the activated Akt signaling induced cardiac hypertrophy in vivo
- VernacularTitle:NF-?B参与Akt信号途径激活诱导的心肌肥大
- Author:
Yuehua LI
;
Tuanzhu HA
;
Qi CHEN
;
Jing LI
;
Chuanf LI
- Publication Type:Journal Article
- Keywords:
Protein kinase B;
NF-kappa B;
Cardiac hypertrophy
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore whether NF-?B activation participates in the activated Akt signaling-induced cardiac hypertrophy in vivo. METHODS: We used two in vivo models of cardiac hypertrophy, namely, aortic banding in Sprague-Dawley rats for 3 weeks and transgenic mice with cardiac specific expression of constitutively active Akt (caAkt). Electrophoretic mobility shift assay (EMSA) was used to determine NF-?B binding activity with nuclear proteins extracted from heart tissues. Western blots were performed to examine the phosphorylation of Akt and phosphor-I?B? with appropriate specific anti-phospho antibodies. RESULTS: ① The heart weight/body weight (HW/BW) ratio was significantly increased by 34 5% ( P
