Proteomic study on the anti-myocardial ischemia mechanisms of Sini decoction
- VernacularTitle:四逆汤抗心肌缺血作用的相关蛋白谱研究
- Author:
Weikang WU
;
Jingping LI
;
Hanchuan LUO
;
Chuanchao XU
- Publication Type:Journal Article
- Keywords:
Sini decoction;
Drugs, Chinese herbal;
Myocardial ischemia;
Proteome
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the mechanisms of the anti-myocardial ischemia of Sini decoction at the level of proteome. METHODS: The total proteins of left ventricular muscle were separated with two-dimensional electrophoresis. The 2-DE images were analyzed with the PDQuest7 1 1 software package. The peptide mass fingerprinting(PMF) was made with MALDI-TOF-MS, then the identification and function of the protein was made via bioinformatics. RESULTS: Sini decoction could regulate the expression of 13 proteins in the ischemic myocardium. The proteins could allocated into five groups: energy metabolism-associated proteins, signal-associated proteins ,self-repair-associated proteins, anti-free radical injury-associated proteins and myocardial contractile-associated proteins. CONCLUSION: Sini decoction could prevent the myocardium from ischemia via regulating expression of different proteins.
