Effect of lactacystin and ?-lactacystin on the activation and proliferation of T-lymphocytes
- VernacularTitle:蛋白酶体抑制剂对T淋巴细胞增殖活化的影响
- Author:
Penghong SONG
;
Haiyang XIE
;
Shusen ZHENG
;
Jian WU
- Publication Type:Journal Article
- Keywords:
Proteasome inhibitors;
T-lymphocytes;
Immunosuppression
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the effects of lactacystin (LAC) and ?-lactacystin (?-LAC), proteasome inhibitor, on the proliferation and activation of T lymphocytes. METHODS: Flow cytometry was used to analyse the proliferation and the expression of CD69, CD25 and CD3 in PHA activated T-lymphocytes. Furthermore, the expression of PA28 and IL-2 mRNA were assayed by competitive RT-PCR. RESULTS: (1) LAC and ?-LAC significantly decreased the incorporation in PHA activated T-lymphocytes. (2) Although LAC and ?-LAC did not affect the expression of CD69 at any time, they significantly inhibited the expression of CD25 (48 h, 72 h, P
