Influence of amylin on apoptosis of human pancreatic islet ?-cells and its molecular mechanism
- VernacularTitle:胰淀素对人胰岛?细胞凋亡的影响及其分子机制的初步研究
- Author:
Dawang WANG
;
Jinxing QUAN
;
Feixia SHEN
;
Jingchen ZHENG
;
Liansong NI
;
Jianb WU
- Publication Type:Journal Article
- Keywords:
Amylin;
Islets of Langerhans;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the molecular mechanism of amylin in inducing apoptosis of human pancreatic islet ?-cells. METHODS: Human pancreatic islet cells were isolated and cultured. The cells were treated with amylin or amylin and aminoguanidine (AG group) for 24 h, respectively. Apoptosis of pancreatic islet ?-cells was studied by in situ TUNEL method combined with double staining for insulin and ELISA. The levels of insulin, NO 2 -/NO 3 - and glutathione (GSH), p53 mRNA and bcl-2 mRNA were also detected. RESULTS: (1) The enrichment factor and the apoptosis rate of pancreatic islet ?-cells in amylin group were markedly higher than that in control group and AG group ( P
