Effects of M. vaccae on eosinophil apoptosis and Bcl-2 protein expression in lung tissues of asthmatic guinea pigs
- VernacularTitle:微卡对哮喘豚鼠肺组织嗜酸粒细胞凋亡及Bcl-2蛋白表达的影响
- Author:
Yunfeng ZHAO
;
Yongai LUO
- Publication Type:Journal Article
- Keywords:
M.vaccae;
Asthma;
Eosinophils;
Apoptosis;
Protein, Bcl-2;
Guinea pig
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of M. vaccae on eosinophil apoptosis and Bcl-2 protein expression in lung tissues of asthmatic guinea pigs. METHODS: 30 guinea pigs were divided into normal saline (NS) group, asthma group and M. vaccae treatment group at random, every group included 10 guinea pigs. Guinea pigs in M. vaccae treatment group were injected intramuscularly with 22 5 ?g M. vaccae 10 days before OVA immunization. TdT-mediated dUTP nick end labeling (TUNEL) technique was used to investigate the apoptosis of eosinophils and immunohistochemistry method was used to study the expression of Bcl-2 protein in lung tissues. RESULTS: The apoptosis index (AI) of eosinophils in lung tissues in M. vaccae treatment group was significant higher than that in asthma group (23 78?5 42)% vs (4 56?0 68)%, P
