Role of intestinal lymphatic pathway in MODS pathogenesis by two-hit in rats
- VernacularTitle:肠淋巴途径在二次打击致大鼠MODS的发病学作用
- Author:
Chunyu NIU
;
Zigang ZHAO
;
Jing ZHANG
;
Gui FAN
- Publication Type:Journal Article
- Keywords:
Multiple organ failure;
Lymphatic system;
Ligation;
Nitric oxide;
Tumor necrosis factor;
Free radicals
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the role of intestinal lymphatic pathway in MODS pathogenesis by two-hit in rats. METHODS: Mesenteric lymph was diverted by ligating mesenteric lymph duct, and the MODS model was established by two-hit method. 45 Wistar rats were divided into three groups: mesenteric lymph duct ligation group, non-ligation group and sham group. All rats facilitated blood withdrawal for serum sample at pre-experiment and after 24 h. Then organs including kidney, liver, lung, heart and intestine were collected for preparing homogenate. The tumor necrosis factor-alpha (TNF?), nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined. RESULTS: It showed that the MODS model was established successfully. After two-hit, the TNF?, NO 2 -/NO 3 -, NOS, iNOS and MDA in serum of non-ligation group were significantly increased than that in pre-experiment and sham group, and SOD was significantly lower ( P
