Effects of NF-?B “decoy” oligodeoxynucleotides on IL-10 expression in LPS-induced mouse macrophages
- VernacularTitle:NF-?B“decoy”寡核苷酸对LPS诱导巨噬细胞IL-10表达的影响
- Author:
Jian WANG
;
Xiaoming DENG
;
Jinbao LI
;
Shengzhon MA
- Publication Type:Journal Article
- Keywords:
Macrophages;
NF-?B decoy oligodeoxynucleotides;
Interleukin-10
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of NF-?B “decoy”oligodeoxynucleotides on IL-10 expression in LPS-induced mouse macrophages. METHODS: Mouse macrophage cell line J774 1 cells were cultured with LPS and liposome-mediated oligodeoxynucleotides, and the level of IL-10 were measured in culture medium by enzyme linked immunosorbent assay. RNA was extracted from macrophages, and the mRNA expression of IL-10 were observed by RT-PCR. RESULTS: NF-?B “decoy”oligodeoxynucleotides did not inhibit the expression of IL-10 in LPS-induced macrophage, and did not reduce generation of IL-10. CONCLUSION: NF-?B decoy ODNs did not depress the mRNA expression of IL-10. NF-?B may not be the critical transcription factor in transduction mechanism of IL-10.
