Effect of benefiting-bone Capsule on IL-6 mRNA and protein expression in rat osteoblasts in vitro
- VernacularTitle:益骨胶囊含药血清对大鼠成骨细胞IL-6 mRNA及其蛋白表达的影响
- Author:
Ronghua ZHANG
;
Keping PENG
;
Xiaofeng ZHU
;
Yu CAI
;
Fen HUANG
- Publication Type:Journal Article
- Keywords:
Benefiting-bone capsule;
Osteoblasts;
Interleukin-6;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The effects of benefiting-bone Capsule (BBC) containing serum on IL-6 mRNA and protein expression in osteoblasts were studied.METHODS: (1) The neonate Sprague-dawley rat osteoblasts were cultured and divided into three groups: group Ⅰ (containing deactivating serum with BBC), group Ⅱ (containing deactivating serum without BBC) and group Ⅲ (DMEM medium group); (2) RT-PCR was used to measure the relative IL-6 mRNA levels; (3) The radioimmunoassay method was used to examine IL-6 protein in the supernatant of the cultured osteoblasts. RESULTS: (1) The relative IL-6 mRNA levels was lower in group I than the control ( P
