Determination of Cilostazol Concentration in Human Plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中西洛他唑的浓度
- Author:
Juying HE
;
Min TANG
;
Peiyuan XIA
;
Songqing LIU
;
Qing DAI
- Publication Type:Journal Article
- Keywords:
Cilostazol;
Concentration in plasma;
HPLC
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To propose a HPLC method for quantitative assessment of cilostazol in human plasma. METHODS: Chromatogram column was C18(150mm?4.6mm, 5?m), mobile phases were acetonitrile-water(40∶60,V/V), the flow rate was 1.4ml/min,determined wavelength was 254nm. RESULTS: Cilostazol concentration was in the linear range of 25～2000ng/ml(r=0.9 999)with the lowest determinable concentration of 12.5ng/ml,recoveries of cilostazol from plasma were 99.99%～101.44%,the relative standard variation of intra-day and inter-day were 0.20%～2.90% and 0.19%～2.13%(n=5) respectively. CONCLUSIONS: This method is s simple, convenient, accurate, and applicable for study of pharmacokinetics of cilostazol in clinic.
