Protective effects of Pinacidil on neurons in rat after anoxic/hypoglycemic damage
- VernacularTitle:吡那地尔对缺氧缺糖损伤神经细胞的保护作用
- Author:
Hua WANG
;
Baofang CHEN
;
Tao LI
- Publication Type:Journal Article
- Keywords:
Pinacidil;
Potassium channels;
Cells, cultured;
Glyburide;
Hypoxia, brain
- From:
Chinese Journal of Perinatal Medicine
1998;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of ATP-sensitive potassium channel (K (ATP)) opener (Pinacidil) on cultured cortical neurons in newborn rats after anoxic/hypoglycemic damage. Methods Neurons were exposed to anoxic/hypoglycemic condition for 4 h,8 h,16 h, after being cultured for 10 days. All cultured neurons were divided into 4 groups: normal control group, model control group, Pinacidil (Pin) group and Pin plus Glibenclamide (Gli) group. The cell mortality, quantity of LDH and the percentage of neuron apoptosis in each group were determined. Results Pinacidil (10~(-6)mol/L ) reduced the percentage of cell death(4 h:21.42?3.68 vs 14.83?2.94; 8 h:36.58?6.14 vs 19.25?3.33; 16 h:49.17?8.3 vs 28.64?6.4, P
