Study on the induction culture and phenotypes of dendritic cells originating from peripheral blood of gastric cancer patients
- VernacularTitle:胃癌患者外周血来源树突状细胞诱导培养及表型分析
- Author:
Kun ZHANG
;
Peiwu YU
;
Pengfen GAO
;
Yun RAO
- Publication Type:Journal Article
- Keywords:
STOMACH NEOPLASMS/blood;
DENDRITIC CELL;
PHENOTYPE
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the morphological and phenotypic characteristics of dendritic cells obtained by induction of peripheral blood monocytes of gastric cancer patients and provide an experimental basis of (dendritic) cells for the biological treatment of gastric cancer. Methods Peripheral blood monocytes of gastric cancer patients were co-cultured with GM-CSF, IL-4 and TNF-? to obtain the dendritic cells. The (morphological) characteristics of the dendritic cells were observed by light and electron microscopes and the (phenotypes) were studied by flow cytometry. Results Mature dendritic cells were oblained seven days after (induction) culture. These cells showed numerous typical dendritic protuberances on their surface.The (phenotypes) of the cells increased gradually with their maturation and reached stable levels seven days later. Conclusions Mature dendritic cells could be obtained from peripheral blood monocytes of gastric cancer (patients) after induction culture with GM-CSF,IL-4 and TNF-?. The obtained cells had typical morphological and phenotypic characteristics. These cells could be used for further research in the biological treatment of (gastric) cancer.
