Role of STAT3 in gut mucosal barrier dysfunction during severe acute pancreatitis
- VernacularTitle:STAT3在急性重症胰腺炎大鼠肠黏膜屏障功能障碍中的作用
- Author:
Jing ZHOU
;
Shan WANG
;
Youzhong AN
;
Yingjiang YE
;
Ming TANG
;
Shen YANG
;
Zhirong CUI
- Publication Type:Journal Article
- Keywords:
Pancreatitis, acute necrotizing;
Intestinal mucosa;
Apoptosis;
Proto-oncogene proteins c-bcl-2;
Proliferating cell nuclear antigen
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the correlation between intestinal epithelial cell apoptosis; and STAT3 activation during murine severe acute pancreatitis (SAP).Methods Wistar rats were divided into four groups (12 in each group): S (sham), C (control), A (SAP), N (NAC was given 1 hour before SAP).Samples were obtained 3 or 24 hours after for the determination of plasma D-lactate, intestinal mucosa MDA and XO, immunohistochemical staining for PCNA.Apoptosis of the epithelial cell was examined with TUNEL and DNA laddering.The expressions of Bcl-2? Bax? p-STAT3 in intestinal mucosa were assessed by Western blot.Results Plasma D-lactate and intestinal epithelial cell apoptosis increased in group A, especially at 24 hours after operation ( P
