Recombinant PML adenovirus suppresses human gallbladder cancer cell growth by inducing apoptosis
- VernacularTitle:腺病毒载体介导的早幼粒细胞性白血病生长抑制因子诱导胆囊癌细胞凋亡的实验研究
- Author:
Aijun ZHU
;
Jingsen SHI
;
Yue HAN
;
Yu REN
;
Lei LI
;
Dalin HE
- Publication Type:Journal Article
- Keywords:
Gallbladder neoplasms;
Adenoviruses, human;
Cell cycle
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism by which promyelocytric leuxemia (PML) suppresses human gallbladder cancer cell line (GBC-SD) growth.Methods GBC-SD cells were infected by green fluorescent protein recombinant adenovirus (Ad-GFP), GFP-positive cells were examined by microscopy.Cultured gallbladder carcinoma cells (GBC-SD) were infected with Ad-PML or Ad-control.Cell death was detected by DNA laddering and TUNNEL analysis.Cell cycle was analyzed by flow cytometer.Results An infection efficiency of 100% can be achieved at a concentration of 100 multiplicity of infection (MOI).The growth rate of the Ad-PML-infected GBC-SD cells was significantly inhibited.DNA laddering was detected at 72 h post-infection.The amount of apoptotic cells significantly increased in the Ad-PML-infected GBC-SD cells without evident alterations in cell cycle distribution.Conclusion PML suppresses growth of human gallbladder cancer cell line GBC-SD by inducing apoptosis.
