Adenovirus mediated fusion gene system driven by KDR promoter kills selectively vein endothelial cells
- VernacularTitle:腺病毒驱动KDR启动子介导CD/TK融合基因系统对血管内皮细胞的靶向杀伤作用
- Author:
Guoqiang SU
;
Zonghai HUANG
;
Jinlong YU
;
Zhou LI
;
Yingfang FAN
;
Huijuan SONG
;
Jinhua ZHANG
- Publication Type:Journal Article
- Keywords:
Endothelium,vascular;
Genes;
Therapeutics;
Adenoviridae
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the selectively killing effect of adenovirus (Ad) mediated double suicide gene driven by KDR promoter on vein endothelial ECV304 cells.Methods KDR producing cells ECV304 and the KDR nonproducing cells LS174T were infected by Ad respectively, followed by treatment with 5-FC and GCV.Killing effects were evaluated and bystander effects were analyzed.Distribution of cell cycle was detected by flow cytometric assay and pathological character of cells was observed by electron microscopy.Results The infection rate of the resultant recombinant Ad to all the cells was not apparently different, and it increased gradually with the addition of multiple of infection (MOI) of Ads.ECV304 cells infected with Ad-KDR-CDglyTK were sensitive highly to the prodrugs, but the LS174T cells infected with Ad-KDR-CDglyTK appeared to be unsensitive to the two prodrugs ( P
