Effects of Zhaoke defibrase and anti-?_v?_3 mAb on the adhesion and immigration of bovine retinal vascular endothelial cells
- VernacularTitle:兆科降纤酶和抗?_v?_3整合素抗体对牛视网膜血管内皮细胞粘附、移行的影响
- Author:
Jing LUO
;
Deyong JIANG
;
Luosheng TANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial cell,eye;
Cell culture;
Antibody;
Adhesion;
Immigration
- From:
Chinese Journal of Ocular Fundus Diseases
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Zhaoke defibrase and anti-? v? 3 mAb (23C6) on the adhesion and immigration of bovine retinal vascular endothelial cells. Methods The culture dishes coated with vitronectin (Vn) and collagen,assays of adhesion and immigration were performed 60 minutes after different concentration of Zhaoke defibrase and anti-? v? 3 mAb was added to the bovine retinal vascular endothelial cells. The apoptosis of bovine retinal vascular endothelial cells induced by Zhaoke defibrase and anti-? v? 3 mAb was detected by electron microscopy. Results Both Zhaoke defibrase and anti-? v? 3 mAb inhibited the adhesion and immigration of bovine retinal vascular endothelial cells in a dose-dependent manner. The inhibited concentration (IC 50) of Zhaoke defibrase was less than 0.05 ?mol/L, while IC 50 of anti-? v? 3 mAb was more than 2.5 ?mol/L. 81.8% endothelial cells adhering to Vn were inhibited by 0 1 ?mol/L Zhaoke defibrase, while 76.3% by endothelial cells adhering to Vn were inhibited by 10 ?mol/L anti-? v? 3 mAb. Typical apoptosis cells were found in bovine retinal vascular endothelial cells after affected by Zhaoke defibrase and anti-? v? 3 mAb. Conclusion Both Zhaoke defibrase and anti-? v? 3 mAb can significantly inhibit the adhesion and immigration of bovine retinal vascular endothelial cells to extracellular matrix, and the mechanism may lie in inducing the apoptosis of endothelial cells.
