Content Determination of Baicalin in Chang'an Capsule by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定肠安胶囊中黄芩苷的含量
- Author:
Jiade SHAO
;
Qinmei ZHOU
;
Xiaobin CHEN
;
Zhengzhong ZHU
;
Yan XU
;
Hong SHEN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Chang’an capsule;
Baicalin;
Determination of content
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for determination of baicalin in Chang’an capsule by RP-HPLC. METHODS:HPLC was performed on C 18 column with methanol-0.07%phosphoric acid solution(44∶56)as mobile phase at a flow rate of lml/min and the temperature of column kept at room temperature.The UV detection wavelength was280nm.RESULTS:The linear range of baicalin was sample size0.1054?g～1.0540?g(r=0.9996),the average recovery was97.44%(RSD=2.92%,n=5).CONCLUSION:The method was simple,accurate and fast,and can be used for the determination of baicalin.
