Content Determination of 2,3,5,4'—tetrahydroxystilbene—2—O—?—D—glucoside in Yangxueshengfa Capsules by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定养血生发胶囊中2,3,5,4’—四羟基二苯乙烯—2—O—?—D—葡萄糖苷的含量
- Author:
Chaofeng LIAO
;
Qingping WU
;
Hongliang XIE
- Publication Type:Journal Article
- Keywords:
Yangxueshengfa capsules;
2,3,5,4'—tetrahydroxystilbene—2—O—?—D—glucoside;
RP-HPLC;
content determination
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the content determination of2,3,5,4'—tetrahydro-xystilbene—2—O—?—D—glucoside in Yangxueshengfa capsules by RP-HPLC.METHODS:Alltima-C 18 was used as the chromatographic column;the acetonitrile-water(15∶85)was taken as the mobile phase with flow speed at1.0ml/min and detection wave?length at320nm.RESULTS:In the range of0.45?g～2.26?g,2,3,5,4'—tetrahydro-xystilbene—2—O—?—D—glucoseide were in direct proportion to the peak area value(r=0.9996),the mean recovery rate was98.32%(RSD=1.58%,n=6). CONCLUSION:This method was simple,convenient and accurate,which can be used for the content determination and the quality control of Yangxueshengfa capsules.
