In Vitro Cultivation of Organ of Corti from Neonatal Rat
- VernacularTitle:新生大鼠耳蜗柯替器体外培养的初步报告
- Author:
Hongyan YUAN
;
Shuhua LI
;
Xingqi LI
- Publication Type:Journal Article
- Keywords:
Rat;
Organ of corti;
Tissue cultivation
- From:
Journal of Audiology and Speech Pathology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish reliable tissue culture methods of organ of Corti in neonatal Wistar Rat.Methods The organ of corti was aquired from Wistar Rat with the method of microdissection.The tissues were cultured by two different methods.Results Twenty-four hours after the tissue cultivation,new epithelia cells and fibroblast were found around the tissue.Inner hair cell、outer hair cell and supporting cell grew well.The tissue was stained by Acridine orange and Prodium iodine.It became green,meaning the tissue had good activity.Conclusion The culture of organ of corti we introduced is an a ideal method for otology research.
