Gene transfer into different passages of bone marrow mesenchymal stem cells
- VernacularTitle:重组腺病毒转染大鼠骨髓间质干细胞的研究
- Author:
Yutao GUO
;
Xiaoying LI
- Publication Type:Journal Article
- Keywords:
Adenoviruses;
Gene transfer;
Gene expression;
Bone marrow;
Mesenchymal stem cells
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05). Flow cytometry analysis by diff erent gates showed the transfection ratio was high in BMSCs in the period of pro d uctive metabolism. The mRNA expression of CAR in P3, P6 and P8 was similar, and the same change was observed in the protein expression of CAR in P3 and P8 BMSCs . CONCLUSION: Ad-CMV-GFP is transferred to BMSC effectively and sustained about 28 days. It is suspected that BMSCs in mitotic phase are easy to be transferred by Ad-CMV-GFP and different passages of BMSCs from P3 to P8 BMSCs can be as high-effectively g ene vehicle.
