Induction of connective tissue growth factor mRNA expression by advanced glycosylation end products in cultured rat renal mesangial cells
- VernacularTitle:糖基化终产物对大鼠肾系膜细胞结缔组织生长因子作用的研究
- Author:
Guihua ZHOU
;
Cai LI
;
Chunsheng MIAO
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Glycosylation end pro ducts, advanced;
Connective tissue growth factor
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of advanced gly cosyla tion end products (AGEs) on connective tissue growth factor (CTGF) mRNA expressi on and extracellular matrix (ECM) synthesis in cultured rat renal mesangial cel ls. METHODS: Rat renal mesangial cells were cultured in vitro un der standard conditions. The content of fibronectin (FN) and type Ⅳ collagen (ColⅣ ) were determined with ELISA after stimulation by AGE-BSA and BSA, respectively. CTGF mRNA expression was assessed by RT-PCR. RESULTS: CTGF mRNA was significantly increased by AGE-BSA in cul tured mesangial cells in a time-and dose-dependent manner compared with BSA cont rol (P
