Study on the activation of blood platelets by propylene- acidamide grafted polypropylene membrane in vitro
- VernacularTitle:一种新型生物人工肝膜材料体外激活血小板的实验
- Author:
Chenghong PENG
;
Zhiming ZHAO
;
Hong LIU
;
Baosan HAN
;
Yong WANG
;
Heqing FANG
;
Yulian WU
;
Changyou GAO
- Publication Type:Journal Article
- Keywords:
Liver, atificial;
Blood compatibility;
Bioco mpatibile materials;
Blood platelets
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the blood compatibility of a new bioartificial reactor membranous material (propylene-acidamide grafted poly propylene membrane, PP-g-AAm) in vitro. METHODS: Contacted PP-g-AAm membrane and PP (polypropylene) memb rane with platelet-rich plasma in a swing bed, 37 ℃, to simulate the conditions in vivo, and another group of PRP without any membranes was set as control group. ELISA was used to study the expression of ?-thromboglobulin, and flow cy tometry was used to study CD62P and CD63 expressio n of the activated blood platelets after contacting the two kinds of membranes w ith PRP. Scanning electrical microscopy was used to study the configuration and numbers of platelet cells adhered on the membranes. RESULTS: After contacting with PRP 30 min, ?-TG expression show ed marked difference between the two kinds of material groups and the control gr oup (P
