Effect of fraction F of naja naja atra venom on protein tyrosine phosphorylation in platelet activation
- VernacularTitle:中华眼镜蛇毒F组份对血小板活化时蛋白酪氨酸磷酸化的影响
- Author:
Mei ZHANG
;
Qingsheng YU
- Publication Type:Journal Article
- Keywords:
Cobra venoms;
Platelet aggregation;
Blotting, Western;
Protein-tyrosine-phosphorylation;
Signal transduction
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the mechanism of intracellu la r signal transduction that fraction F of naja naja atra venom inhibits plate let aggregation. METHODS: Tests were divided into six groups: (1) blank group; (2 ) control group and (3)-(6) ADP plus fraction F group (doses of fraction F were 100, 30, 10, 3 mg/L, respectively). Protein tyrosine phosphorylation in platelet s was assayed by Western blotting and platelet aggregation was assayed by nephel omete r. RESULTS: Fraction F significantly inhibited molecular masses (MW ) 76, 66 and 37.5 kD protein tyrosine phosphorylation in platelet that induced by ADP in a dose-dependent manner, in which 30 and 100 mg/L dose group showed ob viously different effects when compared to control group (P
