Effect of nitric oxide synthase inhibitor aminoguanidine on amino acid contents in ischemic rat brain
- VernacularTitle:一氧化氮合酶抑制剂氨基胍对脑缺血大鼠脑组织氨基酸含量的影响
- Author:
Huixin ZHANG
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Nitric oxide;
Nitric-oxide sy nthase;
Aminoguanidine;
Amino acid
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: By evaluating the effect of selective ni tric oxide synthase inhibitor, aminoguanidine (AG), on the contents of amino aci ds in the rat brain following cerebral ischemia, to investigate the effect of AG on cerebral ischemic injury and the possible mechanism. METHODS: Adult male SD rats, weighing 250-300 g, were matched ra ndomly. We made the focal cerebral ischemic model with thread embolism of left m iddle cerebral artery occlusion (MCAO). AG was administrated after MCAO. Rats we re killed at the scheduled time. The changes of infracted volume and the content s of aspartate, glutamate, glycine and ?-aminobutyric acid (GABA) from striatum , hippocampus and cortex were assayed, respectively. RESULTS: The cerebral infarct volume in AG group were significan tly lower than that in ischemic group. The contents of aspartate, glutamate, gly cine and GABA from striatum, hippocampus and cortex in ischemic group were marke dly higher than those in sham group. The contents of aspartate and glutamate in AG group were significantly lower than those in ischemic group, but the contents of glycine and GABA in AG group were higher than those in ischemic group. CONCLUSION: It may be concluded that AG has a beneficial effect on ischemic rat brain. The possible protective mechanism is that AG can reduce t he contents of aspartate and glutamate, increase the contents of glycine and GAB A.
