Effects of L-carnitine on apoptosis in cultured neonatal rat cardiomyocytes induced by hypoxia/reoxygenation
- VernacularTitle:左旋卡尼汀对缺氧/复氧诱导的子鼠心肌细胞凋亡的影响
- Author:
Yong YANG
;
Guoliang JIA
;
Wenyi GUO
;
Rongqing ZHANG
;
Qing ZHANG
;
Jingxia LI
;
Haokao GAO
- Publication Type:Journal Article
- Keywords:
Carnitine;
Anoxia;
Cardiomyocytes;
Free ra dicals;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine the effects of L-carnitine on apoptosis in cultured neonatal rat cardiomyocytes induced by hypoxia/reoxygenat ion and its possible mechanism. METHODS: A cell culture model of neonatal rat cardiacmyocytes wa s used. The cultured cardiomyocytes were classified into three groups: control g roup, I/R group (anoxia for 120 min, reoxygenation for 240 min) and L-carnitine group (L-carnitine, which was classified into four different concentrations, was added to the cells 2 h before anoxia). The activities of superoxide dismutase ( S OD), the content of malondialdehyde (MDA) and the apoptosis were determined by f low cytometry (FCM). RESULTS: In I/R group SOD activities were lower, and the apoptos is rate and MDA were higher than those in control group and they were statistica lly significant (P
