Inhibitory effect of hypoxia preconditioning on hypoxia/ reoxygenation-induced apoptosis in cardiomyocytes
- VernacularTitle:缺氧预适应抑制缺氧/复氧诱导心肌细胞凋亡的作用及机制
- Author:
Feng ZHANG
;
Qibing MEI
;
Tao ZHANG
;
Yunxin CAO
;
Rutao WANG
;
Chen LI
- Publication Type:Journal Article
- Keywords:
Hypoxic preconditioning;
Myocardium;
Cells;
Apoptosis;
Caspase 3;
Protein Bcl-2
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of hypoxia precondit io ning (HP) in hypoxia-reoxygenation (HR)-induced apoptosis in neonatal rat cardio myocytes and the possible mechanisms. METHODS: Cultured neonatal rat cardiomyocytes were divided into three groups: normal group, HP+H/R group and H/R group. Acridine orange (AO) sta ining was performed to detect morphological changes of apoptotic cells. Apoptosi s rates of cardiomyocytes were detected by flow cytometry. Colorimetric assay wa s used to detect caspase-3 activity. Expression of Bcl-2 protein was detected by immunohistochemistry combined with computer image analysis. RESULTS: Apoptotic cells were detected by AO staining after hypo xia of 6 h followed by 3 h-reoxygenation. The hypodiploid apoptotic peak was det ected by flow cytometry with the apoptotic rates of (29.7?5.4)%. A significan tly reduced apoptotic rates of (7.8?1.3)% was detected in HP group(P
