Influence of allogeneic cornea on human peripheral blood T lymphocyte subtype in vitro
- VernacularTitle:异体角膜刺激引起的人外周血T细胞及其亚群CD25分子表达
- Author:
Jingxiang ZHONG
;
Jintang XU
;
Lian LIU
;
Chen LI
;
Chunyun WU
;
Tongshu ZHENG
;
Dongping SHAO
- Publication Type:Journal Article
- Keywords:
Cornea;
T-lymphocytes;
Receptors, interleuki n-2;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the immunoregulation of all ogeneic cornea on the human peripheral blood T lymphocytes in vitro. METHODS: After Co-culture of human peripheral blood lymphocytes and allogeneic cornea in vitro, T lymphocytes were labeled by monoclonal ant ibody, and analyzed by fluorescent activated cell sorter (FACS). RESULTS: CD25 expression on T lymphocytes in control was 25.2%, after stimulated by the allogeneic cornea or PDB, CD25 expression on T lymphocy tes was 56.8% and 80.9%, respectively. After stimulated by the allogeneic corn ea, CD25 expression on CD 4+ or CD 8+ T lymphocytes were 67.3% and 52.3% , respectively. CONCLUSION: Allageneic cornea stimulates CD25 expression on huma n peripheral blood T lymphocytes, and the CD25 expression on CD 4+ T lymphocy tes is more prominent than CD 8+ T lymphocytes.
