Concentration Determination of Sodium Valproate in Human Serum by HPLC-Fluorescence Detection Method
- VernacularTitle:高效液相色谱-荧光检测法测定人血清中丙戊酸钠浓度
- Author:
Yimin CUI
;
Peihong SUN
;
Yuwang LIU
;
Xia ZHAO
;
Rong LI
;
Zhongmin SUN
- Publication Type:Journal Article
- Keywords:
Sodium valproate;
Steady state trough concentration;
HPLC-Fluorescence
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of sodium valproiate in human serum and to study the bioequivalence of steady state concentration C ssm in of the domestic and imported sodium valproiate sustained-release compound tablets.METHODS:Two periods of multi-oral administration of domestic and imported sodium valproiate sustained-release compound tablets were conducted alternately at random on20healthy male volunteers;the trough concentration of sodium valproate in human serum was determined by HPLC-fluorescence detection and the data were analyzed by3p97pro?gram.RESULTS:The blood concentration was steady after3d oral administration of both the domestic and imported sodium valproate sustained-release compound tablets.The C ssm in of domestic and imported products were(38.17?9.36)、(35.48?9.44)mg/L respectively.C ss min of domestic and imported sodium valproate sustained-release compound tablets were of bioe?quivalence either single or multi-oral administration.CONCLUSION:This HPLC-fluorescence method is quick,sensitive and economical,which can be used to monitor the concentration of sodium valproate in human serum.
