The Changes of Gene Expression Profiling in HL-60 Cells Treated by Chan'su
- VernacularTitle:蟾酥作用后HL-60细胞基因表达谱变化的研究
- Author:
Jiaming TANG
;
Anwei CHEN
;
Kaiping QI
- Publication Type:Journal Article
- Keywords:
Leukemia;
Gene expression profiling;
Chan'su;
Apoptosis;
DNA microarray
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of gene expression profiling in HL-60 cells treated by Chan'su, and the possible mechanisms of Chan'su-induced apoptosis in HL-60 cells. Methods Apoptosis was determined by morphology and agarose gel electrophoresis of DNA fragmentation. The changes of gene expression profiling were detected with DNA microarray. Results 1%(v/v) Chan'su could obviously induce HL-60 cells apoptosis. In the tested 1152 genes, mRNA expression levels of 9 genes changed prior to apoptosis in Chan'su-treated HL-60 cells. Of the 9 genes, 8 gene mRNA levels were up-regulated and 1 gene mRNA level was down-regulated. Conclusion Apoptosis in HL-60 cells treated by Chan'su was involved in many genes. Bcl-2 down-regulation and IL-8 up-regulation were closely correlated with the Chan'su-induced HL-60 cells apoptosis.
