Effects of Melatonin on the Influx of Ca~(2+) Induced by L-glutamate in Cultured Rat Neurons
- VernacularTitle:褪黑素对谷氨酸所致大鼠神经细胞Ca~(2+)内流的影响
- Author:
Hongying YANG
;
Mengliang JIA
;
Chun DONG
- Publication Type:Journal Article
- Keywords:
Melatonin;
L-glutamate;
Neuron;
Calcium ion(Ca 2+ )
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the inhibitory effect of melatonin(MT)on the increased influx of Ca 2+ induced by L-glutamate in cultured rat neurons.METHODS:Fura-2/AM,a Ca 2+ sensitive fluorescent indicator was used to load the brain cells isolated from new-born rats,and the intracellular dissociative Ca 2+ concentration(〔Ca 2+ 〕i)was measured with fluores?cence-spectrophotometer.RESULTS:The L-glutamate of concentration500?mol/L could markedly promote the influx of Ca 2+ and intracellular free Ca 2+ concentration(P
