Content Determination of Three Components in Gentamicin with Scan-TLC
- VernacularTitle:薄层扫描法测定注射用硫酸庆大霉素中3组分的含量
- Author:
Zhong JIA
- Publication Type:Journal Article
- Keywords:
Gentamicin Injection;
Scan-TLC;
Determination of contents
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a rapid determination method for gentaminin active components.METHODS:Scan-TLC procedure was applied.A mixture of methanol-chloroform-28%ammonium hydroxide(1∶1∶1)was taken as developing reagent and ninhydrin in a mixtrue solvent as reveal reagent.The detecting wavelength was500nm.RESULTS:The amounts of three components in gentamincin per spot were linear in the range of2?g～12?g(r 1 =0.9993、r 2 =0.9996、r 1a =0.9991).CONCLUSION:This scan-TLC technique is recommendable for determination of gentamincin components.
