Retinal cells of rat neonates induce the bone marrow stromal stem cells into retinal neurons
- VernacularTitle:新生乳鼠视网膜细胞诱导骨髓间质干细胞向视网膜神经样细胞分化
- Author:
Keming YU
;
Jian GE
;
Jing ZHUANG
;
Huiyi CHEN
;
Zhichong WANG
;
Ling YU
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal stem cells;
Inducing differentiation;
Retina
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the inducing action of retinal cells of rat neonates on the adult rat bone marrow stromal cells (BMSCs).METHODS: The full marrow from adult Wistar rat were cultured and passaged repeatedly to harvest the pure BMSCs. In vitro, the BMSCs were induced by retinal cells of rat neonates in transwell.The morphological changes of the BMSCs were observed under phase contrast microscope, the specific markers of the induced cells were ~identified immunocytochemically with nestin, Map-2, neuron-specific enolase(NSE),neurofilament(NF), Thy 1.1 and glial fibrillary acidic protein(GFAP) antibodies. The mRNA of nestin, NF, NSE, Thy 1.1 and Ran were detected in the induced cells by RT-PCR.RESULTS: The BMSCs were induced by reinal cells of rat neonates into the retina-like neurons, which showed the typical neural morphology and expression of the specific retinal neural antigens.CONCLUSION: The BMSCs from the adult rat can be induced into retina-like cells by retinal cells of rat neonates. [
