Characteristics of bax, bcl-2 and p53 gene expression in children and fetal skin at different developmental stages
- VernacularTitle:不同胎龄的胎儿和少儿皮肤中bax,bcl-2和p53基因表达的变化
- Author:
Wei CHEN
;
Xiaobing FU
;
Shili GE
;
Gang ZHOU
;
Duyin JIANG
;
Tongzhu SUN
;
Bing HAN
;
Zhiyong SHENG
- Publication Type:Journal Article
- Keywords:
Genes, bax;
Genes, bcl-2;
Genes p53;
Fetal skin;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate gene expression of bax, bcl-2 and p53 in fetal skin at different gestational ages and children skin in order to explore their potentially biological significance. METHODS: Apoptosis in skin specimens was determined by terminal deoxynucleotidy transferase mediated dUTP-biotin nick-end labeling technique (TUNEL). Gene expressions of bax, bcl-2 and p53 in skin at different developmental stages was examined with reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Along with fetal growth and development, the incidence rate of apoptosis was increased progressively in skin. In skin from early gestational fetus, bcl-2 was strongly expressed. This gene expression was progressively decreased with increment in gestational age. In children skin, the mRNA content of this gene was significantly reduced compared with fetal skin (P
