NT-3 genetically modified Schwann cells promote neural stem cells to differentiate into neuron-like cells
- VernacularTitle:NT-3基因修饰施万细胞促进神经干细胞体外分化为神经元样细胞
- Author:
Jiasong GUO
;
Yuanshan ZENG
;
Haibiao LI
;
Wenlin HUANG
;
Ranyi LIU
- Publication Type:Journal Article
- Keywords:
Stem cells;
Schwann cells;
Neurotrophin 3;
Neuron-like cells
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of neurotrophin-3 (NT-3)-genetically modified Schwann cells (NT-3-SCs) on differentiation of neural stem cells (NSCs) into the neuron-like cells. METHODS: The NSCs were co-cultured with NT-3-SCs. Report gene LacZ genetically modified Schwann cells (LacZ-SCs) and normal SCs respectively in vitro. 7 d later, the differentiation of NSCs was studied by immunohistochemistry, and the percentage of neuron-like cells was calculated. RESULTS: NSCs differentiated to the GFAP-positive cells (glial-like cells) and NF-positive cells (neuron-like cells) in vitro. Compared to the normal SCs, NT-3-SCs more efficiently promoted NSCs to differentiate into the neuron-like cells. The effect of LacZ-SCs was as the same to the normal SCs. CONCLUSION: NT-3-SCs promote NSCs to differentiate into the neuron-like cells. [
