Alterations of calcium channel gene expression and function in rat ventricular myocytes infected by virus
- VernacularTitle:病毒感染大鼠心室肌细胞钙通道基因表达及功能改变的研究
- Author:
Miao TIAN
;
Shujun HUANG
;
Yuhua LIAO
;
Jihua DONG
;
Min WANG
;
Heping GUO
;
Ming TANG
- Publication Type:Journal Article
- Keywords:
Coxsackievirus infections;
Myocardium;
Calcium channels;
Rats;
Reverse transcription-polymerase chain reaction;
Patch clamp techniques;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the changes of the L-type calcium channel subunit expression and calcium currents (I_~Ca -L) in cultured rat ventricular myocytes infected by coxsackie virus B_3 (CVB_3). METHODS: Primary cultured neonatal rat ventricular myocytes were infected with CVB_3. The changes of L-type calcium channel subunits mRNA in normal and infected myocytes were measured by semi-quantitative reverse transcription-polymerase chain reaction. I_~Ca -L was recorded in two groups respectively using whole cell patch-clamp techniques. RESULTS: The expression of ?_1 and ? subunits of L-type calcium channel mRNA increased in the infected group compared with the normal one (4.00?0.07 vs 2.21?0.41, P0.05). The average current density of I_~Ca -L significantly increased by CVB_3 infection [(-8.66?0.99) pA/pF vs (-6.97?1.75) pA/pF, P
