Potential of human bone marrow mesenchymal stem cells differentiating into hematopoietic cells with fetal liver stromal cell-conditioned medium in vitro
- VernacularTitle:胎肝条件培养液体外诱导人骨髓间充质干细胞定向分化为造血细胞的研究
- Author:
Guanmei WEN
;
Haowei LI
;
Qingzhong XIAO
;
Zhenguang CHEN
;
Xiuming ZHANG
;
Yan LI
;
Xiaodong NA
;
Peng XIANG
;
Shunong LI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Fetal liver
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the potential of human bone marrow mesenchymal stem cells (hMSCs) differentiating into hematopoietic cells with murine fetal liver stromal cell-conditioned medium (FLSC-CM) in vitro. METHODS: 12.5-14.5 days post coitus (dpc ) of KM mice were used for the preparation of fetal liver stromal cell-conditioned medium (FLSC-CM) and embryonic fibroblast feeder layer (FD). Culture-expanded hMSCs were directly contacted with FLSC-CM, FD, and the combination of human interleukin-6 (IL-6) or stem cell factor (SCF), respectively. Seven days later, the non-adherent cells were collected and characterized by morphology, immunophenotypes, and colony forming unit-granulocyte/macrophage culture assay. RESULTS: The number of nonadherent cells derived from hMSCs cultured with FLSC-CM was increased remarkably than those with either FD or cytokines. The non-adhered cells with the morphology of monocyte-or small lymphocyte-like cells were positive for human CD34, CD45 and had the capacity to form the hematopoietic progenitor colonies in methylcellulose cultures containing recombined human granulocyte/macrophage-colony stimulating factor (rhGM-CSF). CONCLUSION: hMSCs were successfully induced toward their differentiation into CD34+CD45+ hematopoietic progenitors after being cultivated with FLSC-CM. This study suggests that hMSCs have the hematopoietic differentiation potential in vitro. [
