Expression of SCL gene in bone marrow stromal cells from normal individuals and patients with aplastic anemia
- VernacularTitle:干细胞白血病基因在再障和正常骨髓基质细胞中的表达
- Author:
Zhen WANG
;
Yangqiu LI
;
Xiuli WU
;
Shaohua CHEN
;
Lijian YANG
;
Yuan ZHANG
;
Kanger ZHU
;
Zhongchao HAN
- Publication Type:Journal Article
- Keywords:
Gene expression;
Stromal cells, bone marrow;
Anemia, aplastic
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of SCL (stem cell leukemia) gene in bone marrow stromal cells (BMSCs) and bone marrow hematopoietic cells from patients with aplastic anemia (AA) and normal individuals. METHODS: Bone marrow stromal cells from AA (9 cases) and normal individuals (33 cases) were amplified by long-term in vitro culture. The adherent and nonadherent cells were collected respectively. RT-PCR-ELISA assay was then performed to detect the expression of SCL gene and the housekeeping gene ?_2 microglobulin (?_2M). The expression ratio of SCL gene were analyzed and its expression level was normalized by ?_2M gene acting as an internal calibration for the purpose of semi-quantitative analysis. RESULTS: The expression ratio of SCL gene was lower in BMSCs from AA (22.2%) than that in normal controls (69.7%, P
